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Digital
Video Database
Digitize
Your Lecture
Create
a custom DVD to accompany your lab manual. Our DVD database
includes video on lab safety, microscope use, Gram staining,
pH testing, pipetting, diffusion and osmosis, respirometer
use, and more. Video clips can be combined in any order
to match your lab manual, or customized with new video created
just for you. Adding content to existing DVD material or
writing new DVD script can earn you, your department, or
your school royalties. The professional videographers at
Bent Tree Press can shoot video at your location, or use
our biologists to shoot video for you—all at incredible
cost-saving pricing!
VIEW
CLIPS OF OUR DVD VIDEOS:
Microbiology
• Biology • Marine
Biology
Microbiology
- Lab
Safety
Covers
basic lab safety, including use of fire extinguisher and
fire blanket, location of First Aid kit, use of eyewash
station, disinfection of workstation, disposal of sharp
and contaminated materials in the SHARPS and biohazard
containers, and location of MSDS information.
-
Parts
of the Microscope
Identifies
and discusses uses of the ocular eyepieces, diopter ring,
rotating nosepiece, objective lenses, stage, condenser,
condensure apeture diaphragm, daylight filter, base, light
source, X & Y travel controls, fine and course focus
knobs, arms, and power switch.
- Use
of the Microscope
Shows
proper transport and setup of microscope, cleaning of
ocular and objective lenses, placement of slide, magnification,
adjustment of eyepiece plates, proper focusing procedure,
and oil immersion viewing and focus, as well as microscope
cleanup and storage.
- Aseptic
Technique
Covers
labeling a culture, turning on and adjusting the Bunsen
burner, flaming the loop, transferring organisms from
a broth, and inoculating an Agar slant.
- Smear
Preparation
Demonstrates
the steps in making a bacterial smear from both broth
and Agar, including aseptic technique for obtaining and
transferring bacteria to a slide, drying a slide, and
properly heat-fixing a slide.
- Simple
Stain
Shows
how to stain a prepared slide and provides examples of
how a correctly stained slide looks under oil immersion,
as compared to stains that are too thick or too thin.
Discusses how to avoid sampling error, and provides examples
of proper interpretation of a stain.
- The
Gram Stain
Provides
history of the Gram stain and discusses positive and negative
results obtained from the procedure. Shows the 4 reagents
used in the Gram stain, and discusses their roles in the
staining process, as well as how to avoid sampling error.
Demonstrates the steps of the staining procedure and shows
examples of correctly stained slides, as well as interpretation
of slides.
- Streak
Plate Technique
Discusses
reasons for using dilution technique and shows labeling,
T-streaking, examples of properly and improperly streaked
cultures, and how to avoid sampling error.
- Serial
Dilutions Using Serological Pipettes
Shows
the setup and labeling of test tubes, aseptic technique
for obtaining a pipette, attaching a pipetting device,
aspirating liquid, filling test tubes with water and broth,
mixing and serial transferring of liquid from one tube
to another, and disposing of used pipettes. Shows labeling
of sterile Petri dishes, transferring broth from test
tubes to culture dishes, and steps in proper spread plate
technique.
- Serial
Dilutions Using Micropipettors
Shows
setup and labeling of microcentrifuge tubes, and micropipetting
of water and broth into each tube. Demonstrates mixing
and serial transferring of liquid from one tube to another.
Shows labeling of sterile Petri dishes, transferring of
broth from test tubes to culture dishes, and proper spread
plate technique.
Biology
- Using
the Computer
Shows
how to turn on the Apple eMac computer, log in, and gain
Internet access. Discusses how to obtain course information
and use weekly links buttons for Web-based lab information.
Shows how to turn off the computer at the end of lab.
- Safety
Discusses
lab safety rules for food and clothing, lab procedures
for backpacks and phones, emergency phone and phone number
location in the lab, as well as eyewash station location
and use, First Aid kit location, fire extinguisher location
and use, and biohazard material disposal.
- Introduction
to the Microscope
Shows
proper transport and setup of the microscope, and identifies
the oculars, stage, objectives, course and fine focus
knobs, slide clip, stage manipulators, power switch, dimmer
dial, and iris.
- Using
the Microscope
Demonstrates how to place a slide, adjust the
objective lenses and eyepieces, focus under low power,
increase magnification, and adjust with the fine focus.
Shows proper technique for preparing the microscope for
storage and transporting it back to the shelf.
- Obsertainer
Discusses what an obsertainer box is, where to
locate the box number, and how to use the obsertainer
in lab.
- Cheek
Cell Preparation
Shows how to clean and prepare a microscope slide,
apply methylene blue dye, obtain a cheek cell scraping,
apply a coverslip to avoid bubbles, dispose of the used
coverslip, and clean and store the slide for future use.
- pH
Testing
Discusses pH and the pH scale, and shows how
to test a sample and read a pH strip.
- Cell
Chemistry: The Test for the Presence of Starch
Demonstrates how iodine reacts with starch in
a potato, and shows how to prepare test tubes and unknowns
for the presence of starch and two other biomolecules.
Shows how to setup and conduct the experiment for the
starch assay and how to perform the Benedict’s test
for sugar and the Biuret test for protein.
- Enzymes
Shows how to prepare and label a spot plate for
the amylase enzyme test. Demonstrates how to add iodine
to each well and test for starch breakdown in the wells
over time. Shows how to time and take samples and read
results, including negative and positive controls. Discusses
variation in results due to heating, cooling, diluting,
or changing pH.
- pH
Effect on Enzymes
Shows the effect of exposing the enzyme amylase
to acid, and demonstrates the spot plate test with the
altered enzyme.
- Diffusion
and Osmosis
Discusses the movement of molecules by diffusion
and osmosis and demonstrates the effect with red dye,
water, and temperature. Demonstrates how to prepare dialysis
tubing with soaking, folding, clipping, and filling. Shows
how to tare the dialysis tubing and perform the experiment.
- Cellular
Respiration: Respirometers
Shows how to make a respirometer by filling and
inverting two differently sized test tubes. Demonstrates
how to prepare three tubes for the experiment, including
the negative and positive controls, by adding the yeast,
water, and glucose to the tubes. Shows the instructor
adding the toxic sodium azide and the addition of phenol
red. Demonstrates inversion of the experimental respirometers.
- Fermentation
Tubes
Demonstrates the steps for testing the effectiveness
of various respiratory fuels, and shows proper technique
for working with a fermentation tube.
Marine
Biology
- Laboratory
Safety
Covers
basic lab safety, including use of fire extinguisher and
fire blanket, location of First Aid kit, use of eyewash
station, disinfection of workstation, disposal of sharp
and contaminated materials in the SHARPS and biohazard
containers, and location of MSDS information.
- Introduction
to the Microscope
Shows
proper transport and setup of the microscope, and identifies
the oculars, stage, objectives, course and fine focus
knobs, slide clip, stage manipulators, power switch, dimmer
dial, and iris.
- Making
Smears of Bacteria
Demonstrates
the steps in making a bacterial smear from both broth
and Agar, including aseptic technique for obtaining and
transferring bacteria to a slide, drying a slide, and
properly heat-fixing a slide.
- Streak
Plate Technique
Discusses
reasons for using dilution technique and shows labeling,
T-streaking, examples of properly and improperly streaked
cultures, and how to avoid sampling error.
- Oil
Immersion Technique
Demonstrates proper focusing procedure: adjustment of
the iris diaphragm, condenser and rheostat, application
of oil and rotation of oil immersion objective to minimize
bubble formation, use of fine focus knob, as well as microscope
cleanup.
- The
Gram Stain
Provides
history of the Gram stain and discusses positive and negative
results obtained from the procedure. Shows the 4 reagents
used in the Gram stain, and discusses their roles in the
staining process, as well as how to avoid sampling error.
Demonstrates the steps of the staining procedure and shows
examples of correctly stained slides, as well as interpretation
of slides.
- Examples
of Zooplankton
Shows locomotion, behaviors, and morphological details
of 17 living species of coastal mero- and holoplankton,
concluding with 7 review questions. Onscreen questions
prompt students to identify locomotory structures and
consider their adaptive significance.
- Making
Pressings of Seaweeds
Demonstrates the proper techniques of mounting and labeling
algae specimens on herbarium paper.
- Sponge
Filtration
Shows excurrent water flow through a large single osculum
on a live sponge after the animal was injected with carmine
solution. An onscreen timer allows students to estimate
filtration rate.
- External
and Internal Anatomy of a Cephalopod
Provides an overview of dissection procedure. Demonstrates
how to make the initial incision & remove the ink
sac. Explains how cephalopod appendages are numbered,
and directs students to find the hectocotylus arm. Demonstrates
removal of skin to make a wet mount for microscopic analysis
of chromatophores, and shows how to open the buccal mass
and isolate the radula.
- Squid
Congregation
Shows nighttime reproductive aggregation of Pacific Market
Squid (Loligo), featuring swimming and mating
behaviors. Striking displays of chromatophore color change
are seen, along with large expanses of the squid's egg
masses attached to the sea floor. The reproductive biology
of Loligo is described, and a bat ray (a squid
predator) is seen swimming through the school.
- External
and Internal Anatomy of a Crab
Provides an overview of dissection procedure. Shows the
exterior morphology of a crab, how to remove the carapace
and hypodermis, and reveals the internal skeletal mechanism
that opens and closes the chelipeds. Footage of living
marine arthropods shows movement of maxillipeds and incurrent/excurrent
respiratory water flow in the blue crab Callinectes;
gill ventilation movements and the coordinated motions
of chelicerae and gnathobases during feeding in the Horseshoe
crab Limulus; burrowing and use of antennae by
the mole crab Emerita (the ventral surface of
a gravid female shows its modified appendages for burrowing).
Remaining video segments show locomotion in juvenile and
adult decorator crabs, and body shapes of 4 other crab
species. Onscreen prompts direct students to think about
structure / function relationships when comparing the
external modifications of the various marine arthropods.
- Sea
Urchin Laboratory
Demonstrates how to safely remove an urchin attached to
the walls of an aquarium, shows injection procedure and
gamete collection, and how to prepare a dilute sperm solution
for fertilization experiments.
- Sea
Urchin Processing Center
A visit to a seafood processing company in San Diego reveals
how uni (a type of sushi) is prepared from the Pacific
red sea urchin. Basic natural history and internal anatomy
is discussed as we follow all stages of the production
process (extraction, washing, draining, sorting, and packing).
- External
and Internal Anatomy of a Bony Fish
Identifies the fins, urogenital opening, and anus, and
shows the sequence of incisions for opening the abdominal
cavity. Demonstrates removal of abdominal fat from internal
organs, and use of probe to identify the stomach.
- Oceanography
Activities on a Pier
Demonstrates several typical sampling and field analysis
procedures for collecting oceanographic data (water sampling,
nutrient testing, determining salinity and dissolved oxygen
level, preparation of BOD collection bottles for transit,
use of Secchi disk and plankton net).
- Fouling
Community Study
Shows dockside collection of epifaunal invertebrates.
Samples are shown being sorted in laboratory. Footage
identifies compound tunicates, shows byssal gland of mussel,
and shows water circulation through a transparent solitary
tunicate. Color images are provided of all the invertebrates
shown in the Fouling Community chapter of the lab manual.
- Sandy
Beach Studies
Demonstrates procedures for conducting an elevational
beach profile, using 5-meter and 1-meter increments; shows
sand sample collection along the transect line, and demonstrates
how to collect meiofauna from beach sand.
- Examples
of Meiofauna
Identifies the main body regions of a rotifer, showing
feeding currents. Shows body flexibility and locomotion
in ciliates, gastrotrichs, nematodes, oligochaetes, and
tardigrades. Identifies the tardigrade stylet. Onscreen
questions invite students to consider which locomotory
mechanisms are employed by the various species.
- Behavior
of Shorebirds
Provides a virtual bird watching session for students
to practice their observational skills on seven coastal
bird species (Pacific Coast) as the birds forage, preen,
and engage in interspecific antagonistic behaviors. Onscreen
prompts ask students to compare and contrast behaviors
seen. Video concludes with review questions to summarize
observations.
- Teacher’s
Modules: For each of the modules below, 15–18
examples (species, external or internal anatomical structures)
are shown for students to identify.
- Plankton
Quiz: 18 species of coastal plankton.
- Squid
Dissection Quiz: 17 anatomical structures.
- Crab
Dissection Quiz: 16 anatomical structures.
- Sea
Urchin Anatomy Quiz: 16 anatomical structures.
- Fish
Dissection Quiz: 15 anatomical structures.
- Footage
of Marine Invertebrates
20 minutes of silent footage show prey capture
and feeding, burrowing, locomotion behaviors in 47 species
from 7 phyla. Time-lapse footage speeds up slow movements,
making them more obvious to see.
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