Introduction
to Cell Culture
Some
Basic Methodologies
- Passage
of Cultures
- Generating
New Plates
- Counting
of Cells via Hemacytometer
- When
to Split and How to Seed
- Passage
Number and Cryogenic Storage of Cells
Exercise
1: Introductory Laboratory: Basic Handling and
Passage of Cells in Culture
Exercise
2: Influence of Serum, Coating Surfaces and Alteration
in Signal Transduction on Cell Division and Differentiation
Exercise
3: Cryogenic Storage of Cells
Exercise
4: Immunocytochemisty
Exercise
5: Generation of Primary Cultures from Mouse
Embryos
Exercise
6: Transient Transfection of Mammalian Cells
Exercise
7: Migration and Colony Formation of Endothelial
Cells
Exercise
8: Determination of the Cell Cycle
Exercise
9: Chromosome Analysis Lab Introduction to Plant
Cell Culture
Exercise
10: Comparison of Root Tip Squashes Grown in
Different Sterile Conditions
Exercise
11: Organogenesis and Callus Formation from African
Violet Leaves
Exercise
12: Corn Embryo Culture
